Increase in local protein concentration by field-inversion gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Pulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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By making use of preparative field inversion gel electrophoresis, we have constructed a lambda ZAP library that is highly enriched for sequences from the choroideremia locus. In vivo excision of pBluescript SK(-) constructs from lambda ZAP obviates the subcloning of DNA inserts and allows for rapid processing of several hundred recombinants. From a 625 kb Sfil fragment we isolated 7 clones that...
متن کاملEpidemiologic study of Taylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments.
Contagious equine metritis (CEM), a sexually transmitted bacterial disease, was first described in thoroughbred horses. It also occurs in nonthoroughbred horses, in which it produces isolated, apparently unrelated outbreaks. Thirty-two strains of Taylorella equigenitalis, the causative agent of CEM, from all over the world were characterized by field inversion gel electrophoresis of fragments o...
متن کاملMolecular Typing of Acinetobacter Baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis
Background & Objective : Currently, Acinetobacter baumannii is an important nosocomial pathogen insofar as its hospital outbreaks have been described from various geographical areas. Since the discrimination of strains within a species is important for delineating nosocomial outbreaks, this study was conducted with the aim of genotyping the A. baumannii clinical strains in Tehran via the pulsed...
متن کاملFalse-negative urine protein electrophoresis by semiautomated gel electrophoresis.
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually 50-fold) followed by manual application of the concentrated sample to a gel. Recently, semiau...
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ژورنال
عنوان ژورنال: Proteome Science
سال: 2007
ISSN: 1477-5956
DOI: 10.1186/1477-5956-5-18